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Invitrogen
Rat IgA Uncoated ELISA Kit with Plates
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Product Specifications
Analytical sensitivity
16 ng/mL
Assay range
16-1,000 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Supernatant
50 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
10 x 96 Tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Storage
2-8°C
Protein name
Rat IgA
Target site-specificity
Assay kit format
Uncoated ELISA Kit with Plates
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Rat Immunoglobulin A (IgA) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IgA. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Rat IgA is an essential immunoglobulin isotype that is predominantly present in mucosal secretions like colostrum, saliva, and tears. It acts as a crucial defense mechanism against pathogens that enter the body through mucosal surfaces. Rat IgA is made up of two heavy chains and two light chains, with a molecular weight of approximately 170 kDa. It is produced by B cells in response to antigenic stimulation and has the ability to bind to various antigens, including bacteria, viruses, and toxins. Rat IgA functions by neutralizing these pathogens and preventing their attachment to mucosal surfaces, which helps to reduce the risk of infection. It also activates complement and recruits immune cells to the site of infection, further enhancing the immune response. Rat IgA is widely used as a research tool in immunology and infectious disease studies and has been produced for therapeutic applications such as in the treatment of inflammatory bowel disease and mucosal infections. Overall, Rat IgA is a critical component of the immune system and plays a crucial role in protecting the body against mucosal infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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