IgG (Total) Human Uncoated ELISA Kit with Plates

Catalog number:  88-50550-22

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

IgG is the major immunoglobulin in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid and a key player in the humoral immune response. Serum IgG in healthy humans presents approximately 15% of total protein beside albumins, enzymes, other globulins and many more.

The Fc portion of IgG, but not F(ab')2 or Fab fragments, can cross the placenta of a mother to enter the fetal circulation providing the fetus with postpartum protection.

IgG molecules are able to react with Fc-gamma receptors that are present on the surfaces of macrophages, neutrophils, natural killer cells, and can activate the complement system.

The binding of the Fc portion of IgG to the receptor present on a phagocyte is a critical step in the opsonizing property IgG provides to the immune response. Phagocytosis of particles coated with IgG antibodies is a vital mechanism to cope with microorganisms.

IgG is produced in a delayed response to an infection and can be retained in the body for a long time. The longevity in serum makes IgG most useful for passive immunization by transfer of this antibody. Detection of IgG usually indicates a prior infection or vaccination.

Suitable Sample Types
Cell culture supernatant, serum and plasma

Sample Volume
100 µL (prediluted 1:500,000)

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: 1.6-100 ng/mL
Assay Sensitivity: 1.6 ng/mL
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Ready-SET-Go!, eBioscience
Gene ID: Non-Gene
Gene Symbol: Non-Gene
Immunoassay Kit Format: Uncoated ELISA Kit with Plates
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Name: Immunoglobulin G
Sample Type (Specific): Cell Culture Supernatants, Plasma, Serum
Sample Volume: 100 µL (prediluted 1:500,000)
Target (Kit Named): IgG
Target Species (Named): Human
Target Species (Validated): Human
Target Sub-Specificity: Total

Contents & storage

Capture Antibody Concentrate
Detection Antibody Concentrate
Human IgG isotype control (standard), lyophilized, 200 ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Tetramethylbenzidine (TMB) Substrate Solution
96-well plates included with product Cat. Nos. ending in suffixes -22, -76, -86

Documents

Manuals & protocols