IFN gamma Mouse Uncoated ELISA Kit with Plates

Catalog number: 88-7314-22

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

This Mouse IFN gamma Uncoated ELISA Kit with Plates contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of mouse IFN gamma protein levels from samples including serum, and supernatants from cell cultures.

Special Note: To ensure optimal results from the Mouse IFN gamma Uncoated ELISA Kit with Plates, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: 15-2,000 pg/mL
Assay Sensitivity: 15 pg/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: Ready-SET-Go!, eBioscience
Gene Aliases: IFN-g, Ifg
Gene ID: 15978
Gene Name: interferon gamma
Gene Symbol: IFNG
Immunoassay Kit Format: Uncoated ELISA Kit with Plates
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Aliases: IFN-gamma
Protein Name: Interferon gamma
Target (Kit Named): IFN gamma
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P01580

Contents & storage

Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -44, -76, -86)

Documents

Manuals & protocols