High Select™ Phosphopeptide Enrichment Kits & Reagents

Thermo Scientific™

High Select™ Phosphopeptide Enrichment Kits & Reagents

Name: High Select™ Phosphopeptide Enrichment Kits & Reagents
SKU: 88303
Price: 2109000 KRW

The expanded High Select family of complete kits supports enrichment of phosphorylated peptides using Fe-NTA and TiO₂ immobilized resins in spin column, tip, and magnetic bead formats designed for use with MS analysis.

보기 방식 변경

카탈로그 번호	수량	제품 유형
88303	96 Tips	TiO₂ Phosphopeptide Enrichment Spin Tips
A32992	30 Reactions	Fe-NTA Phosphopeptide Enrichment Kit
A32993	24 Reactions	TiO₂ Phosphopeptide Enrichment Kit
88302	30 Columns	Graphite Spin Column

4 개 옵션

카탈로그 번호 88303

제품 가격(KRW)

2,109,000

온라인 행사

Ends: 30-Jun-2026

2,481,000

할인액 372,000 (15%)

Each

수량:

96 Tips

제품 유형:

TiO₂ Phosphopeptide Enrichment Spin Tips

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

2,109,000

온라인 행사

Ends: 30-Jun-2026

2,481,000

할인액 372,000 (15%)

Each

Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionization, and incomplete fragmentation of phosphopeptides. Given the low relative abundance of phosphorylation modifications in complex protein samples, enrichment is essential for successful MS analysis of phosphopeptides. High-Select Fe-NTA and TiO₂ phosphopeptide enrichment kits complement our lysis, reduction, alkylation, and digestion reagents, along with our C18, graphite spin, and high-pH reversed-phase peptide fractionation columns to provide a complete workflow for phosphopeptide enrichment.

Convenient—pre-formulated buffers for ease-of-use in spin-column (Fe-NTA and TiO₂) or magnetic (Fe-NTA) formats to enable parallel processing of multiple samples
High specificity—recovers phosphopeptides with > 90% selectivity
Superior recovery—enriches more total and unique phosphopeptides than other commercially available resins
High coverage—recovers peptides with single and multiple phosphorylation sites
Scalable—compatible with automation, including the use of KingFisher magnetic particle processors

High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest as starting sample. Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150 μg of phosphopeptides with greater than 90% selectivity in less than 45 minutes.

High-Select TiO2 Phosphopeptide Enrichment Kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides. Each column can enrich phosphopeptides from 0.5 to 3 mg of total protein digest as starting sample. The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns. Use of this kit results in enrichment of up to 150 μg of phosphopeptides with greater than 90% selectivity. TiO2 enriches a unique set of phosphopeptides that complements the High-Select Fe-NTA IMAC Phosphopeptide Enrichment Kit.

For high resolution analysis of the enriched phosphopeptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat. No. ES902 or ES903). The stand-alone High Select Fe-NTA Magnetic Agarose beads provide flexibility for scale and throughput and may be used in both manual and automated platforms, including KingFisher 96 and KingFisher Flex magnetic particle processors.

For Research Use Only. Not for use in diagnostic procedures.

사양

최종 제품 유형Peptides

용도(장비)Microcentrifuge, Mass Spectrometer

수량96 Tips

워크플로우 단계Sample Enrichment

검출 방법Mass Spectrometry

형식Spin Column

제품라인Pierce

제품 유형TiO₂ Phosphopeptide Enrichment Spin Tips

시작 물질Peptides, Protease-digested Protein

Unit SizeEach

구성 및 보관

Store at 4°C.

자주 묻는 질문(FAQ)

In the SMOAC protocol (https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/10/High-SelectTM-SMOAC-Protocol.pdf), can I enrich with High-Select Fe-NTA kit first?

No. It is important to enrich with the TiO2 kit (Cat. No. A32993) first. Afterwards, the flow-through and wash fractions from this enrichment can be processed with the Fe-NTA kit (Cat. No. A32992). If this order is reversed (that is, Fe-NTA before TiO2), there will be 2 consequences as follows: 1. There will not be any significant additional recovery of peptides (maybe just a few more peptides). 2. There will be no enrichment for the multiple phosphorylated peptides, so those would be lost.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why do you offer two phosphopeptide enrichment kits: the High-Select TiO2 kit (Cat. No. A32993) and the High-Select Fe-NTA kit (Cat. No. A32993)?

The two phosphopeptides enrichment kits, Fe-NTA and TiO2, enrich a complementary set of phosphopeptides. Our R&D has developed a Sequential enrichment of Metal Oxide Affinity Chromatography (see https://assets.thermofisher.com/TFS-Assets/CMD/posters/PO-65032-SMOAC-Phosphoproteomics-Peptides-ASMS2017-PO65032-EN.pdf and https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/10/High-SelectTM-SMOAC-Protocol.pdf?CID=bid_mic_r04_jp_cp0000_pjt0000_bid00000_0so_blg_protein_analysis_mass_spectrometry_bid_ts_mbr_24065_Social_LAB) where flow-through and wash fractions from TiO2 enrichment were combined and subjected to Fe-NTA enrichment. This sequential enrichment provides impressive coverage of phosphoproteomes.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the differences between the old Fe-NTA kit (Cat. No. 88300) and the new High-Select Fe-NTA kit (Cat. No. A32992)?

There are four differences between the Fe-NTA kit (Cat. No. 88300) and the new High-Select Fe-NTA kit (Cat. No. A32992) kit as follows: 1. The selectivity - the ratio of number of phosphopeptides over total peptides - was significantly improved to 99% with Cat. No. A32992, because the reagents were extensively optimized for the phosphopeptide selection. 2. The phosphopeptide yield was also increased to 33 µg based on quantitative colorimetric peptide assay (Cat. No. 23275). 3. The reagent is a pre-formulated format, so mixing reagent to prepare the working solution from stock solutions provided in the old kit (Cat. No. 88300) is not necessary, so it is easier to handle. 4. The enrichment protocol is optimized and streamlined, which means there are many fewer steps than with Cat. No. 88300. Thus, it takes <45 min to finish the entire protocol compared to 2 hours with the old kit (Cat. No. 88300).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.