HiPPR™ Detergent Removal Spin Columns, 0.1 mL, 24 x 0.1 mL Columns - FAQs

View additional product information for Detergent Removal Spin Columns & Plates - FAQs (87779, 88307, 87777, 88306, 87778, 88305, 88304, 87776, 87780)

2 product FAQs found

I still have detergent present in my sample after protein digestion. Is there anything I can do?

Some detergents can be removed from peptide samples using Pierce Detergent Removal Resin (Cat. No. 87780) or HiPPR Detergent Removal Spin Columns (Cat. No. 88306) in the HIPPR (High Protein and Peptide Recovery) format. However, detergents are more easily removed at the protein level using acetone precipitation, dialysis, or affinity capture using Pierce Detergent Removal Resin and HiPPR Detergent Removal Spin Columns before protein digestion.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the best way to remove detergents from my sample for mass spectrometry analysis?

Detergents are more easily removed at the protein level using acetone precipitation (https://tools.thermofisher.com/content/sfs/brochures/TR0049-Acetone-precipitation.pdf), dialysis, or affinity capture using Pierce Detergent Removal Resin (Cat. No. 87780) or HiPPR Detergent Removal Spin Columns (Cat. No. 88306).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.