Pierce™ 6 Protein Digest, equimolar, LC-MS grade - FAQs

The UniProt IDs for the proteins in the Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342) standard, are:
    Serum Albumin, Bovine (Uniprot: P02769)
    Cytochrome C, Bovine (Uniprot: P62894)
    Lysozyme, Chicken (Uniprot: P00698)
     β-galactosidase, E. coli (Uniprot: P00722)
    Apo-Transferrin, Bovine (Uniprot: Q29443)
    Alcohol Dehydrogenase, S. cerevisiae (Uniprot: P00330)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center or Mass Spectrometry Support Center.

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

We recommend using the moderately complex Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.