Pierce™ NHS-Activated Magnetic Beads, 5 mL - FAQs

View additional product information for Pierce™ NHS-Activated Magnetic Beads - FAQs (88827, 88826)

2 product FAQs found

Can you provide the shelf-life for NHS-Activated Magnetic Beads?

NHS-Activated Magnetic Beads is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do to increase the coupling efficiency of antibodies to the Pierce NHS-Activated Magnetic Beads?

-Make sure all primary amine-containing buffer components of the buffer like Tris or glycine are completely removed by dialysis, desalting, or the use of the Pierce Antibody Clean-Up Kit (Cat. No. 44600) before coupling to magnetic beads
-Make sure the antibody is mixed with the NHS-beads IMMEDIATELY after washing the beads. A delay may cause hydrolysis of the NHS groups on the resin which leads to low coupling efficiency.
-Finally make sure the pH of the non-amine-containing coupling buffer used for coupling is between pH 7-9

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.