Pierce™ Biotin 3' End DNA Labeling Kit

Catalog number: 89818

Thermo Scientific™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting | Nucleic Acid Labeling & Oligo Synthesis

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Description

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 20 reactions

Contents & storage

Sufficient For: 20 labeling reactions (each with 5 pmol DNA)
• 1 mL 5X TdT Reaction Buffer (-20°C)
• 5 µL Terminal Deoxynucleotidyl Tranferase (-20°C)
• 1 µL Biotin-11-UTP (≤4°C)
• 14 µL Unlabeled Control Oligo (-20°C)
• 4 µL Biotin-Control Oligo (-20°C)

Documents

Manuals & protocols

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