In-Gel Tryptic Digestion Kit

Catalog number: 89871

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

Thermo Scientific Pierce In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Features of the kit:

Convenient—includes all necessary reagents for destaining coomassie- or fluorescent dye-stained proteins, reduction and alkylation of cystines, and tryptic digestion
Robust—the procedure and reagents produce reliable digestions and data generation using a wide range of conditions without optimization
Accurate—contains highly purified and modified MS-grade trypsin that shows no chymotryptic activity and minimal autolytic activity

The In-Gel Tryptic Digestion Kit provides a complete set of reagents for performing approximately 150 digestions on colloidal coomassie- or fluorescent dye-stained protein bands. The kit includes modified porcine trypsin, destaining buffers, reduction reagents, alkylation reagents and digestion buffers along with detailed and simple instructions. Each component and step has been optimized and balanced to produce complete, accurate and clean digests using a variety of conditions for dependable proteomics analysis of peptide profiles by mass spectrometry (MS).

Accurate identification and analysis of proteins is a central component of proteomic strategies for studying cellular functions and processes. Mass spectrometers are essential tools for these studies as they provide a high level of sensitivity and mass accuracy. However, this sensitivity and accuracy can be obtained only if proper sample preparation is performed. Two key sample preparation steps are the fragmentation of proteins into peptides and subsequent concentration and clean up of peptides. The In-Gel Tryptic Digestion Kit accomplishes the first of these steps. Thermo Scientific Pierce C-18 Columns accomplish the second step.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 150 reactions
Final Product: Peptide
Workflow Step: Protein Digestion
Detection Method: Mass Spectrometry
Starting Material: Gels, Protein samples, Cell lysate
For Use With (Equipment): Mass Spectrometer

Contents & storage

Upon receipt store Pierce Trypsin Protease, MS Grade at -20°C in a nonfrost-free freezer. Store all other components at 4°C. Product shipped on gel pack. See Important Product Information for additional storage and handling information for each component upon receipt.

Documents

Manuals & protocols