Pierce™ Albumin/IgG Removal Kit

Description

The Thermo Scientific Pierce Albumin/IgG Removal Kit is optimized to decrease the abundant albumin and antibody components of human serum sample in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

Features of the Albumin/IgG Removal Kit:

• Optimized for 2D sample prep—microcentrifuge spin cup method is scaled and optimized for treating 10 µL human serum samples for 2D electrophoresis
• Efficient—kit removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels
• Fast—kit processes samples in less than 40 minutes
• Scalable—use different amounts of affinity resin to match sample volume and concentration

This dye/Protein A-based kit uses a mixture of Cibacron Blue Dye and Protein A agarose affinity resin. The dye binds to albumin with relatively good specificity, and Protein A binds to many species and subclasses of IgG. The Thermo Scientific Pierce Albumin/IgG Removal Kit is economical and can be used to treat samples from other species besides human (e.g., monkey, swine, rabbit). The kit is also scalable and sufficient to process 25 samples, each containing 600µg of total serum protein (about 10µL of serum).

Low-abundant proteins in human serum and plasma can provide information about human diseases. However, human fluid analysis is often complicated by the presence of high concentrations of albumin and IgG, which can make up more than 70% of total serum protein (Figure, Panel A). The Thermo Scientific Pierce Albumin/IgG Removal Kit uses a classical mixture of Cibacron Blue Dye and Protein A resin for economical removal of albumin and IgG. This kit can process up to 25 samples of approximately 600 µg of serum using an optimized protocol to minimize nonspecific protein interactions and sample dilution (Figure, Panel C).

Figures

Specifications

Column Type:	Affinity, Agarose Resin
Format:	Kit, Microcentrifuge, Spin Column
Immobilized Phase:	Cibacron™ Blue Dye, Protein A
Product Line:	Pierce™
Product Size:	25 reactions

Contents & storage