Thermo Scientific™

Pierce™ Albumin Serum Depletion Kits

Catalog number: 89875
Have Questions?
Thermo Scientific™

Pierce™ Albumin Serum Depletion Kits

Catalog number: 89875
Have Questions?
Concentrations of albumin and IgG can make up more than 70% of total serum proteins and often mask lower quantity proteins during protein-profiling experiments. Optimize your serum sample prep by removing albumin or IgG quickly and conveniently. By using our depletion kits to remove excess high-abundance serum proteins, less abundant proteins of interest can be easily detected by 2D electrophoresis and other protein profiling methods such as mass spectrometry.
 
Catalog Number
89875
Unit Size
25 reactions
Description
Albumin and IgG Removal Kit
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionAlbumin and IgG Removal Kit
For Use With (Equipment)Microcentrifuge, mass spectrometer, 2D gel electrophoresis
Product TypeAlbumin/Antibody Removal Kit
Product LinePierce™
Label or DyeCibacron™ Blue Dye, Protein A
Shipping ConditionProduct shipped with an ice pack.
Starting MaterialPlasma, Serum
Final Product TypeProtein
Detection Method2D Gel Electrophoresis, Mass Spectrometry
FormatSpin Column Kit
Workflow StepSample preparation
Quantity25 Reactions
Purification TargetSerum Proteins
Unit Size25 reactions
Catalog Number
Unit Size
24 reactions
Description
Albumin Depletion Kit
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionAlbumin Depletion Kit
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeAlbumin Depletion Kit
Product LinePierce™
Sufficient ForRemoving albumin from 24 human serum samples, 10 to 50 μL each
Starting MaterialPlasma, Serum
Final Product TypeProtein
Detection MethodMass Spectrometry
FormatKit
Workflow StepSample Enrichment, Sample Clean Up
Quantity24 Reactions
Unit Size24 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
89875Full specifications
25 reactionsAlbumin and IgG Removal Kit
-
DescriptionAlbumin and IgG Removal Kit
For Use With (Equipment)Microcentrifuge, mass spectrometer, 2D gel electrophoresis
Product TypeAlbumin/Antibody Removal Kit
Product LinePierce™
Label or DyeCibacron™ Blue Dye, Protein A
Shipping ConditionProduct shipped with an ice pack.
Starting MaterialPlasma, Serum
Final Product TypeProtein
Detection Method2D Gel Electrophoresis, Mass Spectrometry
FormatSpin Column Kit
Workflow StepSample preparation
Quantity25 Reactions
Purification TargetSerum Proteins
Unit Size25 reactions
85160Full specifications
24 reactionsAlbumin Depletion Kit
-
DescriptionAlbumin Depletion Kit
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeAlbumin Depletion Kit
Product LinePierce™
Sufficient ForRemoving albumin from 24 human serum samples, 10 to 50 μL each
Starting MaterialPlasma, Serum
Final Product TypeProtein
Detection MethodMass Spectrometry
FormatKit
Workflow StepSample Enrichment, Sample Clean Up
Quantity24 Reactions
Unit Size24 reactions
Showing 2 of 2
Pierce Albumin/IgG Removal Kit

Optimized—microcentrifuge spin cup method is scaled for treating 10 μL human serum samples for 2D electrophoresis
Efficient—removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels
Fast—processes samples in less than 40 minutes
Scalable—use different amounts of affinity resin to match sample volume and concentration

The Thermo Scientific Pierce Albumin/IgG Removal Kit uses a classical mixture of Cibacron Blue Dye and Protein A resin for removal of albumin and IgG. The dye binds to albumin with good specificity, and Protein A binds to many species and subclasses of IgG. The Pierce Albumin/IgG Removal Kit is economical and can be used to treat samples from other species besides human (e.g., monkey, swine, rabbit). The kit is also scalable and sufficient to process 25 samples, each containing 600 μg of total serum protein (about 10 μL of serum).

Pierce Albumin Depletion Kit

Complete—includes optimized buffer and microcentrifuge spin columns to remove albumin quickly and conveniently from 10- to 50-μL samples
Adaptable—easy-to-dispense slurry improves performance, eases processing of multiple samples, and can be adapted to larger or smaller columns or 96-well filter plates
Compatible—removing excess albumin facilitates MS analysis or electrophoresis gel analysis of low-abundance serum proteins

The Thermo Scientific Pierce Albumin Depletion Kit resin is supplied as a 50% slurry. To prepare the depletion columns, 200 μL of slurry is dispensed into the supplied microcentrifuge spin columns to obtain 100 μL of settled beads to process 10 to 50 μL of serum sample in a single reaction. The kit has been optimized to bind human, porcine, and sheep albumin from serum samples. With a modification to the protocol, albumin from bovine, calf, goat, and rat can be removed with this method. The kit is not effective for removal of mouse albumin. Processed samples are ready for immediate downstream analysis by electrophoresis or MS applications.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Upon receipt, store at 4°C. Do not freeze.

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