TMTsixplex™ Isobaric Label Reagent Set, 2 x 0.8 mg, 2 x 0.8 mg (per tag) - FAQs

View additional product information for TMTsixplex™ Isobaric Label Reagent Set - FAQs (90066, 90068, 90062, 90061)

4 product FAQs found

What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis?

We recommend using high-resolution Orbitrap Tribrid (e.g., Fusion, Lumos, Eclipse), Orbitrap Exploris (e.g., 240, 480), or Q Exactive (e.g,. Plus, HF, HFX) series of instruments.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With the TMTsixplex Isobaric Label Reagent Set (Cat. Nos. 90061, 90062), what is the smallest amount of protein that can be labeled?

The smallest amount of protein that can be labeled is 10 ug, and the volumes for reduction, alkylation, and digestion need to be adjusted. In order to get a good labeling efficiency, we recommend using a peptide: TMT ratio of 1:8.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is TMT quantitation relative or absolute?

TMT quantitation is relative between samples unless a heavy peptide standard is included for comparison.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the main purpose of TMT (Tandem Mass Tag) reagents?

TMT technology is used to perform multiplex quantitation of proteins extracted from cells and tissues. Rather than quantify single proteins (for example with an antibody), here, large numbers of proteins can be quantified in a single run.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.