Where can I download the control sequences for expression arrays?
Sequences of all our controls can be downloaded from the sequence file that is available on product pages on thermofisher.com.
For example, here is the link to the product page for Clariom D Assay, human: https://www.thermofisher.com/order/catalog/product/902923
Go to the "Software & Data Analysis" section and under "Support Files", click on "Sequence Files: Clariom D Array, human Probe Sequences, tabular format", which you can open in Excel.
Note: If the control sequence file is not available, please contact techsupport@thermofisher.com.
What is the shelf life for expression microarrays?
Typical shelf life for expression arrays are as follows:
- All ≥ 8 µm commercial expression cartridges are stable up to 24 months.
- All ≥ 8 µm commercial resequencing cartridges are stable up to 18 months.
- All ≥ 8 µm commercial expression plates are stable up to 18 months.
Please note that this excludes products such as Exon or Gene array as they are 5 µm cartridge and plates.
Does genomic DNA contamination in the sample interfere with the results, and how do I monitor the degree of its effect?
By titrating genomic DNA back into the total RNA samples and monitoring the deterioration of the array data, it was determined during development of the assay that a moderate amount of genomic DNA contamination will only have minimum effect on the array results. Therefore, routine RNA isolation techniques coupled with DNase treatment should yield sufficiently high-quality sample for analysis on the exon arrays.
Find additional tips, troubleshooting help, and resources within our
Microarray Analysis Support Center.