Thermo Scientific™

Pierce™ Lys-C Protease, MS-grade

Catalog number:  90307

Related applications:

Protein Mass Spectrometry Analysis

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Description

Thermo Scientific™ Pierce™ Lys-C Protease, MS Grade is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

Features of Lys-C Protease, MS Grade include:
Enhanced digestion—use in tandem with trypsin to decrease tryptic missed cleavages
Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization, and fragmentation properties
Versatility—effective enzyme activity under highly denaturing conditions (e.g., 8 M urea)
C-terminal lysine cleavage specificity—at least 90% for a complex protein sample
Stability—provided in a lyophilized format

Product details
This is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes. Lys-C protease has high activity and specificity for lysine residues, resulting in larger peptides and less sample complexity than trypsin (i.e., fewer peptides). Unlike trypsin, Lys-C protease can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. These unique Lys-C protease properties ensure high digestion efficiency when used alone or followed by tryptic digestion. Additionally, Lys-C prototypic peptides typically have higher charge states, making it an enzyme of choice for use with ETD fragmentation.

Lys-C protease is commonly used in phosphopeptide enrichment workflows because it generates peptides with primary amines at both the N- and C-terminus, allowing the fragments to be double-labeled with amine-reactive isobaric tags. This results in enhanced peptide ionization and improved limits of quantitation since more fragment ions can be re-isolated during MS3 acquisition. This enzyme can be used for in-solution or in-gel digestion workflows to produce peptides for LC-MS/MS protein identification. This Lys-C enzyme is packaged lyophilized (20 µg or 100 µg quantities).

The endoproteinase Lys-C specifically hydrolyzes proteins at the carboxyl side of lysine. Efficient protein digestion can be completed in 2 hours at 37°C. Lys-C protease remains active in highly denaturing conditions such as 8 M urea, 2 M guanidine-HCl, 1% SDS, 2% CHAPS, and 40% acetonitrile and functions well within pH 7–9 (maximal activity at pH 8). This lyophilized enzyme has a mass of 30 kDa and is stable for 1 year when stored at –20°C.

Applications
• Improved sequence coverage of protein digests
• De novo sequencing
• Epigenetic studies
• In-gel and in-solution digestion of proteins

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product Type: Peptide
For Use With (Equipment): Mass Spectrometer
Product Line: Pierce™
Shipping Condition: Wet Ice
Starting Material: Protein samples, Cell Lysate
Workflow Step: Protein Digestion

Contents & storage

Store in freezer (-5 to -30°C).