Pierce™ MS-Compatible Magnetic IP Kit, protein A/G

Catalog number: 90409

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS
Sensitive—procedure successfully enriches low abundance proteins (low ng range)
Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteins
Robust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per protein

The Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone. The optimized kit components have been formulated to be compatible with downstream LC-MS analysis. After the immunoprecipitation procedure, the target-enriched elution fraction is ready for in-solution tryptic digestion without the need for gel purification, detergent removal, or desalting.

This kit has been rigorously validated using numerous target antigens with varying expression levels, including targets previously undetected without enrichment or by western blotting. Additionally, the reagents and procedures have been validated using both manual and automated magnetic separation.

• Enrichment of low abundance targets to improve MS sensitivity
• Identification of target proteins that are not detectable by western blotting
• Quantitation of target proteins by LC-MS analysis
• Screening of antibodies for IP-MS
• Enrichment of target proteins upstream of targeted analysis (SRM)
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 40 reactions
Final Product: Protein
Workflow Step: Sample Enrichment
Detection Method: Mass Spectrometry
Starting Material: Cell Lysate, Protein samples
For Use With (Equipment): Magnetic Stand, KingFisher™ Flex

Contents & storage

Protein A/G Magnetic Beads, 1 mL
IP-MS Cell Lysis Buffer, 100 mL
IP-MS Wash Buffer A, 75 mL
IP-MS Wash Buffer B, 40 mL
IP-MS Elution Buffer, 6 mL
MgCl2 (1 M), 0.75 mL

Store in refrigerator (2–8°C).


Manuals & protocols

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