Alexa Fluor™ 532 Protein Labeling Kit

Description

The Alexa Fluor 532 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive yellow fluorescent Alexa Fluor 532 dye (ex/em 532/554 nm). This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 532. Comparably small amounts of other proteins (>20 kDa) can also be labeled. Each kit contains everything needed for three labeling reactions and subsequent purification for free dye removal. Our protein labeling kits include an easy-to-use pre-packed spin column for rapid dye removal and high antibody recovery typically greater than 85%. Stabilizing proteins must be removed from the sample before labeling.

Alexa Fluor 532 Protein Labeling Kit features include:
• Convenient—labeled proteins typically ready to use in 90 min (~15 min hands-on time)
• High conjugate recovery—pre-packed spin columns generally result in >85 % recovery of antibody conjugate
• Simple protocol—react, separate, use; detailed instructions included for optional degree of labeling (DOL) determination
• Rapid purification—quickly remove unbound dye with included pre-packed spin columns in under 15 minutes

Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISA, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor dye colors plus Pacific Blue, biotin, Oregon Green 488, Fluorescein-EX, and Texas Red dyes.

Better results and workflows with labeled primary antibodies
A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

The benefits of Alexa Fluor dyes
Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor dyes, higher degrees of labeling can be achieved without intramolecular quenching. Learn more about Alexa Fluor Dyes ›.

Learn more about protein and antibody labeling
We offer a wide selection of antibody and protein labeling kits to fit your starting material and your experimental setup. Learn more about antibody labeling kits and fluorescent protein labeling.

Custom antibody conjugates
If you can’t find what you’re looking for in our stocked list, we can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

Specifications

Detection Method:	Fluorescent
Excitation/Emission:	532⁄553
Flow Cytometer Laser Lines:	532
Label Type:	Alexa Fluor Dyes
Labeling Method:	Conjugation-Based
Labeling Scale:	1 mg
Labeling Target:	Antibodies (General), Proteins (General)
Label or Dye:	Alexa Fluor™ 532
No. of Reactions:	3
Product Line:	Alexa Fluor™
Shipping Condition:	Room Temperature

Contents & storage