Alexa Fluor™ 532 Protein Labeling Kit

Description

Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor™ dye colors, biotin, the hapten Oregon Green™ 488, fluorescein EX, and Texas Red™ dye. Each kit provides the components needed to perform three protein conjugations and purifications.

View a selection guide for all Protein Labeling Kits.

View the Fluorophore Selection Guide.

Important features of protein labeling kits:

• Labeled proteins typically ready to use in 2 hr (~30 min hands-on time)
• Designed to label 1 mg of IgG
• Simple protocol—react, separate, use
• Stabilizing proteins must be removed from the sample before labeling

The benefits of Alexa Fluor™ dyes
Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching.

Learn more about protein and antibody labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

We’ll make a custom antibody conjugate for you
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

Specifications

Detection Method:	Fluorescent
Excitation⁄Emission (nm):	532⁄553
Flow Cytometer Laser Lines:	532
Label or Dye:	Alexa Fluor® 532
Labeling Method:	Conjugation-Based
Labeling Scale:	1 mg
Labeling Target:	Antibodies (General), Proteins (General)
Number of Reactions:	3
Product Line:	Alexa Fluor™
Product Size:	1 kit
Shipping Condition:	Room Temperature

Contents & storage