The red-fluorescent Alexa Fluor® 594 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format
Solid
Quantity
0.5 mg
Product Type
Azide
Solubility
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Chemical Reactivity
Alkyne
Detection Method
Fluorescent
Shipping Condition
Room Temperature
System Type
Click-iT™
Reactive Moiety
Amine, Azide
Label or Dye
Alexa Fluor™ 594
Label Type
Alexa Fluor
Color
Red, Red
Product Line
Alexa Fluor™, Molecular Probes™
Emission
617
Excitation
590
Contents & Storage
Store at ≤-20°C, desiccate and protect from light.