AbC™ Anti-Mouse Bead Kit

Catalog number: A10344

Molecular Probes™  Related applications: Flow Cytometry

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Description

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

Species reactivity—for use with all isotypes of mouse antibody conjugates
Maximum laser compatibility—for use with all lasers

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Mouse Bead Kit was developed to address this issue.

View a selection guide for all flow cytometry compensation beads.

How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Species reactivity
The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins.

Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bead Diameter: 6 µm
Number of Reactions: 100
Stain Configuration: Surface
Flow Cytometer Laser Lines: any lasers (UV to 633/635)
Format: Tube(s)
Product Size: 1 kit
Calibration Type: Flow Cytometry Compensation Beads
Detection Method: Fluorescent
Diameter (Metric): 6 µm
For Use With (Equipment): Flow Cytometer
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial AbC™ capture beads and 1 vial of negative beads.
Store at 2-8°C.

Documents

Manuals & protocols

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