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CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Catalog number: A1048503

Gibco™  Related applications: Cell Isolation & Expansion | Mammalian Cell Culture | Primary Cell Culture

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Description

Gibco® OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium
• Consistent performance in supporting T-cell expansion
• Supports high density cultures in static and WAVE Bioreactor® cultures
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Versatile T Cell Culture Media
OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

Ready for T Cell Expansion
OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x106 cells/mL in static conditions and >2x107 cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

Stability of Complete Media
Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

Simplify Your Transition into Clinical Research
The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco® CTS™ products deliver:
• Reduced burden in qualifying reagents
• Appropriate certificates of analyses and origin included
• Convenient access to our Drug Master File (DMF)

Specifications

Form: Liquid
Glucose: Low Glucose
Species: Human
Quantity: 1 bag
Cell Line: [other]
Cell Type: T Cells
Glutamine: No Glutamine
Sterility: Sterile
Serum Level: Serum-Free
Culture Type: Suspension Cell Culture
HEPES Buffer: HEPES
Product Size: 1,000 mL bag
Classification: Serum-Free, Chemically Defined, Xeno-Free
Endotoxin Level: Very Low
Inorganic Salts: Calcium, Magnesium
Volume (Metric): 1 L
Culture Environment: CO2
Included Antibiotics: No Antibiotics
Phenol Red Indicator: Phenol Red
Purity or Quality Grade: Research Grade
Sodium Pyruvate Additive: Sodium Pyruvate
Sodium Bicarbonate Buffer: Sodium Bicarbonate

Contents & storage

OpTmizer™ T-Cell Expansion Basal Medium (1 x L media bag): Store at 2–8°C. Protect from light.

OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.

Documents

Manuals & protocols