SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays

Catalog number: A10752030

Invitrogen™  Related applications: Microarray Analysis | Nucleic Acid Labeling & Oligo Synthesis

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Description

The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript® II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

System: Affymetrix GeneChip®
Comments: For generating double-stranded cDNA
Label or Dye: Biotin
Product Size: 30 reactions
Final Product: cDNA (Double-Stranded)
Labeling Method: Direct Labeling
Labeling Target: cDNA
Detection Method: Biotin-Based
Includes Label or Dye: No
Sample Type (General): RNA
Sample Type (Specific): Total RNA, Poly(A+) RNA
Shipping Condition: Dry Ice

Contents & storage

50 µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript® II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
All components are guaranteed stable for 6 months when properly stored.

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