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Invitrogen
Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor™ 488
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FIGURE: 1 / 4
Fluorescein/Oregon Green Antibody (A-11090) in ICC/IF
Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with an a-satellite probe labeled with the Oregon Green® 488 dye and specific for chromosome 17. The probe was labeled using the ULYSIS Alexa Fluor® 488 Nucleic Acid Labeling Kit (Product # U21659) (left panel). The signal was amplified using the Alexa Fluor® 488 conjugate of rabbit anti-fluorescein/Oregon Green® an... View More
Product Details
A-11090
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fluorescein/Oregon Green
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
purified
Storage buffer
0.1M potassium phosphate, pH 8
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The Alexa Fluor 488 conjugates can be used to amplify the fluorescence signal while still allowing fluorescein-compatible optics. Excitation 495 nm/peak emission 519 nm.
It is a good practice to centrifuge the labeled antibody solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FITC
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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