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          • Primary Antibodies ›
          • Alexa Fluor 488 Antibodies

          Invitrogen

          Alexa Fluor 488 Polyclonal Antibody

          19 Published Figures
          167 References
          View all (4) Alexa Fluor 488 antibodies

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          Cite Alexa Fluor 488 Polyclonal Antibody

          Additional Information:
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          • Published Figures (19)
          Alexa Fluor 488 Antibody in Western Blot (WB)
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          Alexa Fluor 488 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          Alexa Fluor 488 Antibody (A-11094) in WB

          Cell death & disease 2021 - Fig. 5 MRPS31 suppression induces mitoribosomal dysfunction, OXPHOS defect, and cell invasiveness. MRPS31_high type cells (JHH4 and HepG2) were transfected with MRPS31 siRNA for 3 days. A Western blots. B Western blots after sucrose gradient sedimentation analysis of mitoribosome. MRPS29 (a small subunit protein) and MRPL13 and MRPL48 (large subunit proteins) were used as indicators of each subunit. Purple,... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Alexa Fluor 488 Antibody in Western Blot (WB)
          Alexa Fluor 488 Antibody in Immunocytochemistry (ICC/IF)
          Alexa Fluor 488 Antibody in Immunocytochemistry (ICC/IF)
          Alexa Fluor 488 Antibody in Immunocytochemistry (ICC/IF)
          Alexa Fluor 488 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Alexa Fluor 488 Antibody in Immunocytochemistry (ICC/IF)
          Alexa Fluor 488 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (IHC)
          Alexa Fluor 488 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          Alexa Fluor 488 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          Alexa Fluor 488 Antibody in Western Blot (WB)
          Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
          Alexa Fluor 488 Antibody in Flow Cytometry (Flow)
          Alexa Fluor 488 Antibody in Flow Cytometry, Peptide array (Flow, ARRAY)

          Product Details

          A-11094

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 11 publications 11 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 49 publications 49 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 4 publications 4 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 61 publications 61 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 19 publications 19 publications

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          -
          View 4 publications 4 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 2 publications 2 publications

          Proximity Ligation Assay (PLA) (PLA)

          -
          View 1 publication 1 publication

          Peptide Array (Array)

          -
          View 1 publication 1 publication

          Immunomicroscopy (IM)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (Misc)

          -
          View 7 publications 7 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical, Dog, Fruit fly, Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Alexa Fluor 488 coupled to carrier protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          500 µg

          Purification

          purified

          Storage buffer

          PBS, pH 7.2

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_221544

          Target Information

          Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AF488; Alexa 489; AlexaFluor488

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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