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Invitrogen
alpha Tubulin Monoclonal Antibody (236-10501)
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FIGURE: 1 / 95
alpha Tubulin Antibody (A11126) in ICC/IF
Immunofluorescent analysis of Alpha-Tubulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Alpha-Tubulin Mouse monoclonal Antibody (Product # A11126) at 1 µg/mL in 1% BSA and incubated for 3 hours at room tem... View More
Product Details
A11126
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Published species
Bovine,
Fruit fly,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Plant,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
236-10501
Immunogen
Bovine Alpha-Tubulin.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The anti-bovine alpha-tubulin antibody can be used with secondary detection techniques to visualize microtubules in fixed cells and in fixed or frozen tissue sections of various species. In addition, this antibody can be used to detect tubulin by ELISA and immunoblot, for the screening of expression libraries and, since it recognizes amino acids 65-97 of the N-terminal structural domain, as a probe for the N-terminal domain of alpha-tubulin.
We have found that this highly specific antibody can be used at much lower concentrations than anti-tubulin antibodies available from other suppliers. For immunocytochemistry applications, we recommend using an initial concentration of 1 µg/mL or less.
Dissolve in PBS with 1% BSA and 2 mM sodium azide to obtain a stock concentration of 0.2 mg/mL.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; TUBB; Tubulin alpha; tubulin alpha 6; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2B; tubulin, beta polypeptide; tubulin, beta polypeptide paralog; unnamed protein product
Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; SPAX11; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBB2B
UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Human) Q9BQE3, (Bovine) Q3ZCJ7, (Human) P0DPH7, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) P68366, (Bovine) P81948, (Human) A6NHL2, (Human) Q9BVA1, (Bovine) Q6B856, (Mouse) P68369, (Mouse) P05213, (Mouse) P68373, (Mouse) P68368, (Mouse) Q3UX10, (Mouse) Q9CWF2
Entrez Gene ID: (Bovine) 404053, (Human) 7846, (Bovine) 539882, (Human) 10376, (Human) 84790, (Bovine) 504244, (Human) 7278, (Human) 112714, (Bovine) 534900, (Human) 7277, (Bovine) 777775, (Human) 79861, (Bovine) 538631, (Human) 347733, (Bovine) 281555, (Mouse) 22142, (Mouse) 22143, (Mouse) 22146, (Mouse) 22145, (Mouse) 238463, (Mouse) 73710
GTPase activity
nucleotide binding
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
hydrolase activity
identical protein binding
macromolecular complex binding
metal ion binding
protein heterodimerization activity
double-stranded RNA binding
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
protein domain specific binding
molecular_function
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
neuron migration
startle response
intracellular protein transport
microtubule-based process
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
neuron differentiation
sperm motility
adult behavior
cytoskeleton-dependent intracellular transport
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
cell division
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
cellular response to interleukin-4
cytoskeleton organization
nervous system development
modulation of synaptic transmission
positive regulation of axon guidance
embryonic brain development
protein polymerization
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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