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ViraPower™ HiPerform™ Promoterless Gateway™ Expression System
The ViraPower™ HiPerform™ Promoterless Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cellRead more
| Catalog Number | Quantity |
|---|---|
| A11145 | 20 reactions |
Catalog number A11145
Price (USD)
4,510.00
Each
Quantity:
20 reactions
Price (USD)
4,510.00
Each
The ViraPower™ HiPerform™ Promoterless Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and MultiSite Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The promoterless pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway™ technology
• Expression of the target gene under the control of a promoter of choice
• Stable, long-term expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems
Key Features
• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Promoterless vector to express the target gene under the control of a promoter of choice
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (Cat # A11146)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat # 12538120)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway™ technology
• Expression of the target gene under the control of a promoter of choice
• Stable, long-term expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems
Key Features
• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Promoterless vector to express the target gene under the control of a promoter of choice
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (Cat # A11146)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
• Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat # 12538120)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialAmpicillin (AmpR), Blasticidin (BsdR)
CleavageNo Cleavage Site
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
For Use With (Application)Clone your own Promoter, Viral Expression
Gateway EnzymeLR Clonase™ II Plus, LR Clonase™ II
PCR EnzymeProof-Reading Polymerase
Product TypeExpression System
Protein Tag Position (to your gene)C-terminal
Quantity20 reactions
Replication OriginpUC Origin
Selection Agent (Eukaryotic)Blasticidin
Selection Marker EukaryoticBsdR
Selection Marker PromoterPGK Promoter
Special ElementsWPRE, cPPT
VectorpLenti
Cloning MethodGateway
Concentration2 μg/mL
Product LineGateway, HiPerform, ViraPower
PromoterNone (Promoterless), EF-1α, CMV
Protein TagV5 Epitope Tag
Unit SizeEach
Contents & Storage
Contents
The ViraPower™ HiPerform™ Promoterless Gateway™ Expression System includes the following components:
ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (Cat # A11146) which includes:
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit which includes:
6 ug of pLenti6.4⁄R4R2⁄V5-DEST™ vector (40 μL of vector at 150 ng⁄μL in TE Buffer, pH 8.0) 10 ug of pLenti6.4⁄CMV⁄V5-MSGW⁄lacZ plasmid (20 μL of vector at 0.5 μg⁄μL in TE Buffer, pH 8.0) 10 ug of pENTR™ 5’⁄CMVp plasmid (20 μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0) 10 ug of pENTR™ 5’⁄EF1αp plasmid (20 μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
pENTR™ 5’ TOPO™ TA Cloning Kit (Cat # K59120) which includes:
pENTRâ„¢ 5´-TOPO™ TA Cloning™ Reagents, including pENTRâ„¢ 5´-TOPO™ Vector One Shot™ TOP10 Chemically Competent E. coli (C4040-03) One Shot™ Stbl3™ Chemically Competent E. coli (Cat # C737303): 21 X 50 μL of Stbl3™ Cells, 6 mL of S.O.C. Medium and 50 μL of pUC19 control DNA (10 pg⁄μL) pENTR™ Gus positive control plasmid
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000) which includes:
195 ug of ViraPower™ Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 ug⁄μL in TE Buffer, pH 8.0) 0.75 ml of Lipofectamine-™ 2000 (Cat # 11668027) 50 mg of Blasticidin powder (Cat # R21001) 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106 frozen cells in 1 mL of Freezing Medium Gateway™ LR Clonase™ II Plus Enzyme Mix (Cat # 12538120): 40 μL of Gateway™ LR Clonase™ II Plus Enzyme Mix and 40 μL of Proteinase K Solution at a concentration of 2 ug⁄mL
Storage
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit: -20°C
• pENTR™ 5’ TOPO™ Reagents: -20°C
• ViraPower™ Packaging Mix: -20°C
• LR Clonase™ II Plus Enzyme Mix: -80°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E.coli: -80°C
• One Shot™ TOP10 Chemically competent E.coli: -80°C
• 293FT Cell Line: Liquid Nitrogen
• pENTR™ Gus positive Control: -20°C
The ViraPower™ HiPerform™ Promoterless Gateway™ Expression System includes the following components:
ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (Cat # A11146) which includes:
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit which includes:
pENTR™ 5’ TOPO™ TA Cloning Kit (Cat # K59120) which includes:
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000) which includes:
Storage
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit: -20°C
• pENTR™ 5’ TOPO™ Reagents: -20°C
• ViraPower™ Packaging Mix: -20°C
• LR Clonase™ II Plus Enzyme Mix: -80°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E.coli: -80°C
• One Shot™ TOP10 Chemically competent E.coli: -80°C
• 293FT Cell Line: Liquid Nitrogen
• pENTR™ Gus positive Control: -20°C