Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains the MultiSite Gateway™-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4/R4R2/V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.4/R4R2/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
Key Features
Kit includes
pLenti6.4/R4R2/V5-DEST™ Kit
• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg
• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
Store at -20°C
pENTR 5' TOPO™ TA Cloning Kit (Cat. No. K95120)
• pENTR 5' TOPO™ Reagents, store at -20°C.
• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.
One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL
• pUC19 control DNA (10 pg/μL), 50 μL
Store at -80°C
pENTR™ Gus positive control plasmid, store at -20°C