Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified
Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified
Citas y referencias (7)
Gibco™

Geltrex™ Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem-cell qualified

Geltrex LDEV-free stem cell-qualified reduced-growth factor basement membrane matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-SwarmMás información
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Número de catálogoCantidad
A14133025 mL
A14133011 mL
Número de catálogo A1413302
Precio (USD)
579,96
Each
Añadir al carro de la compra
Cantidad:
5 mL
Precio (USD)
579,96
Each
Añadir al carro de la compra

Geltrex LDEV-free stem cell-qualified reduced-growth factor basement membrane matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV) sometimes found in this type of preparation. Routinely used for attachment and maintenance of human iPSCs or embryonic stem cells, every lot of this matrix is quality control tested using hESCs or iPSCs to help ensure growth and pluripotency of each lot.

Features of Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, include:

  • Every lot qualified using hESC or iPSC, helping reduce the need for customers to lot screen
  • Free of lactose dehydrogenase elevating virus (LDEV), making it safe for all types of cell culture
  • Consistent protein concentration lot-to-lot, helping ensure reproducible results

Gain reliability and consistency in your hESC/iPSC studies

Human hESCs/iPSCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support. Geltrex Reduced-Growth Factor Basement-Membrane Matrix, LDEV-free, stem cell-qualified, has been tested for support of hESC/iPSC growth and pluripotency, minimizing the need to test multiple lots to find one that works. Our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, so you can have confidence in every purchase.

Free of LDEV contamination

Recent advances in processing of EHS tumor cells have resulted in virus-free basement membrane extracts. Every lot is tested by tested by RT-PCR methods so you can be confident that every lot is virus-free.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas madre pluripotentes inducidas humanas (iPSC), células epiteliales primarias, células endoteliales, células de músculo liso
Concentración12-18 mg/mL
Nivel de endotoxinasBajo
FormatoTubo(s)
Línea de productosGibco
Tipo de productoBasement Membrane Matrix
Grado de pureza o calidadCalidad para cultivo celular
Cantidad5 mL
Duración de almacenamiento36 meses
Condiciones de envíoHielo seco
EspecieRatón
ClasificaciónFactor de crecimiento reducido
Culture TypeCultivo celular adherente
FormularioCongelado
Serum LevelSin suero
EsterilidadEstéril
Sin aditivosNo Phenol Red
Unit SizeEach
Contenido y almacenamiento
Almacenar a – 20 °C.

Preguntas frecuentes

Can I use the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) with other PSC media systems (i.e. StemFlex, Essential 8, Essential 8 Flex, etc.)?

No, the Ready-To-Use Geltrex matrix was developed for use with the StemPro hESC SFM System. It is not recommended for other PSC media systems.

For other PSC media systems, we recommend the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement?

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601) is a pre-diluted Geltrex product ready to use for coating culture vessels. There is no need to dilute the solution further. The product has also been QC tested using hESCs to ensure that every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1569601)

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302) is a "concentrated" version of the Geltrex hESC-Qualified, Ready-To-Use above. You can pre-dilute this concentrated solution to pre-coat the culture vessel to grow hESCs at your desired usage concentration. This Geltrex matrix has also been QC tested using hESCs to ensure every lot will maintain the attached pluripotency of these cells. The full product description can be found here:

Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413301, A1413302)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202) is the same as the product above. However, it has not been tested to verify it will support hESC growth and expansion. This is a "general" version and not specific to a cell type or line. A full product description can be found here:

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. Nos. A1413201, A1413202)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I prepare Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) stock solution, and how long is the stock solution stable after thawing?

We recommend thawing Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. 1413301, 1413302) at 4 degrees C before making your stock solution. On ice, make a 1:1 dilution with DMEM/F-12 and aliquot into smaller volumes. Stock solution aliquots should be stored at -20 degrees C or used immediately. Once the stock solution is thawed, it should be used for coating plates immediately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix and Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix? I am working with human hepatocytes.

We do not have comparison data for the two Geltrex products. We recommend using the Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix for hepatocytes. We have not performed a significant amount of testing using the Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix on these cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What substrate should I use for cardiomyocyte differentiation?

We recommend using Gibco Geltrex LDEV-Free, hESC Qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or A1413302) or Gibco Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700) for xeno-free applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Matriz de membrana basal del factor de crecimiento reducido cualificada para hESC y libre de LDEV Geltrex™ FAQs

Citations & References (7)

Citations & References
Abstract
Characterizing Pluripotent Stem Cells Using the TaqMan(®) hPSC Scorecard (TM) Panel.
Authors:Fergus J, Quintanilla R, Lakshmipathy U,
Journal:
PubMed ID:25138722
'Rapid technological developments for the efficient generation of footprint-free induced pluripotent stem cells (iPSC) enabled the creation of patient-specific iPSC for downstream applications in drug discovery and regenerative medicine. However, the large number of iPSCs, generated from diverse genetic backgrounds using various methods and culture conditions, created a steep challenge ... More
CRISPR/Cas9 Genome Editing of Human-Induced Pluripotent Stem Cells Followed by Granulocytic Differentiation.
Authors:Dannenmann B, Nasri M, Welte K, Skokowa J
Journal:Methods Mol Biol
PubMed ID:32006418
'Research on patient-derived induced pluripotent stem cells (iPSCs) could immensely benefit from the implementation of CRISPR/Cas9 genome editing of iPSCs, creating unique opportunities such as the establishment of isogenic iPSC lines for disease modeling or personalized patient-specific drug screenings. Here we describe a stepwise protocol of safe, efficient, and selection-free ... More
A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes.
Authors:Murphy C, Feifel E, Jennings P, Gstraunthaler G, Wilmes A
Journal:Methods Mol Biol
PubMed ID:31124107
'Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an ... More
Kinetochore Proteins Have a Post-Mitotic Function in Neurodevelopment.
Authors:Zhao G, Oztan A, Ye Y, Schwarz TL
Journal:Dev Cell
PubMed ID:30827899
'The kinetochore is a complex of proteins, broadly conserved from yeast to man, that resides at the centromere and is essential for chromosome segregation in dividing cells. There are no known functions of the core complex outside of the centromere. We now show that the proteins of the kinetochore have ... More
Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells.
Authors:Winston TS, Suddhapas K, Wang C, Ramos R, Soman P, Ma Z
Journal:Stem Cells Int
PubMed ID:30766604
Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to
View all Matriz de membrana basal del factor de crecimiento reducido cualificada para hESC y libre de LDEV Geltrex™ citations