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TaqPath™ 1-Step RT-qPCR Master Mix, CG, 5 x 1 mL - FAQs

View additional product information for TaqPath™ 1-Step RT-qPCR Master Mix, CG - FAQs (A15300, A15299)

2 product FAQs found

What is the recommended RNA input range for TaqPath 1-Step RT-qPCR Master Mix, CG (Cat. Nos. A15299, A15300)?

The figure for TaqPath 1-Step RT-qPCR Master Mix, CG (Cat. Nos. A15299, A15300) below, shows the dynamic range of RNA Triplex Amplification at 0.1 pg–100 ng. While a reaction volume is not specified, we typically use 20 µL reactions.
RNA Triplex Amplification

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What starting template can I use with the TaqPath 1-Step RT-qPCR Master Mix?

You can use this protocol for both RNA and DNA targets. During thermal cycling, the reverse transcription step will not affect performance with DNA targets.