SeCore® DRB1 Locus Exon 2 & 3 Sequencing Kit (IVD)

Catalog number: A15573

Invitrogen™  Related applications: Diagnostic Testing

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The SeCore® DRB1 Locus Exon 2 & 3 Sequencing Kit is a set of reagents for directly identifying DNA sequence from target HLA DRB1 loci. This kit enables complete bidirectional sequencing of Exon 2 & 3 for DRB1.

Benefits of the SeCore® DRB1 Locus Exon 2 & 3 Sequencing Kit include:

• Increased confidence with complete bidirectional sequencing
• Ease of analysis of the first hypervariable region
• Helps reduce time for ambiguity resolution
• Preparation of all Class II with same protocol

Complete bidirectional sequencing of Exon 2 & 3 allows for confidence in your results. It also provides a redundancy of information that minimizes the need for repeat testing. Because the amplification primers are located outside of Exon 2, you are able to see clean sequence in both directions of the first hypervariable region. By sequencing both Exon 2 & 3, you can reduce your need for additional methods to resolve ambiguities within those regions. SeCore® Class II products all follow the same protocol.

What You Get
The SeCore® DRB1 Locus Exon 2 & 3 Sequencing Kit consists of two main components:

• The Pre-PCR box includes two amplification mixes, one for Exon 2 and one for Exon 3, plus FastStart™ Taq DNA Polymerase.
• The Post-PCR box includes ExoSAP-IT® reagent, 5 ready-to-use sequencing mixes containing the sequencing primers, dye terminators and sequencing enzyme, and Sequencing PPT Buffer.

The kit contains enough material for approximately 25/500 tests.

The SeCore® HLA Sequencing System is for the identification and definition of Class I and II Human Leukocyte Antigens (HLAs) and is for use in HLA typing. The SeCore® HLA Sequencing System provides human histocompatibility information of HLA Class I (A, B, and C) and Class II (DPB1, DQB1, and DR) loci using genomic DNA isolated from whole blood specimens. HLA typing using the SeCore® Sequencing Kits must be performed in the presence of an HLA Lab Director, Technical Supervisor and/or general Supervisor following accepted laboratory accreditation standards (ASHI). These products are for professional use only. This test must not be used as the sole basis for making clinical decisions. For the intended use of individual system components, refer to the documentation provided with the component.

Contents & storage

• 2 vials Amplification Mix
• 1 vial FastStart™ Taq DNA Polymerase
• 1 vial ExoSAP-IT®
• 5 vials Sequencing Mix
• 1 vial Sequencing PPT Buffer

Store at -5 to -30°C.


Manuals & protocols