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TaqMan® hPSC Scorecard™ Panel, 384-well

Catalog number: A15870

Applied Biosystems™  Related applications: Stem Cell Analysis

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Description

The Applied Biosystems® TaqMan® hPSC Scorecard™ Panel 384w enables verification of pluripotency and determination of lineage bias for both human ES and iPS cell lines. The 384-well plate contains four sets of 94 predefined TaqMan® Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is not included with this panel. For a kit that combines the panel with master mix, see TaqMan® hPSC Scorecard™ Kit 384w. This product is also available in a 2 x 96w Fast format.

• Assess the gene signature of four samples in one simple experiment
• Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software
• Identify lines with trilineage differentiation potential
• Evaluate inductive differentiation specified to one germ layer
• Access validated content for increased confidence in your results

Assess an Entire Gene Signature in One Experiment
The TaqMan® hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan® Master Mix to your cDNA of interest and transfer to the 384-well plate with a multichannel pipette.

Compare Results to a Reference Standard
The TaqMan® hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems® qRT-PCR systems and is available at no additional charge.

Identify Lines with Trilineage Differentiation Potential
Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

Evaluate Inductive Differentiation Specified to One Germ Layer
The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

Access Validated Content
The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

What You Get
The TaqMan® hPSC Scorecard™ Panel 384w includes:

• One 384-well plate of 94 TaqMan® Gene Expression assays (4 gene sets per plate)
• One optical plate cover
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Dried Down
Format: 384-well plate
Species: Human
Product Size: 1 x 384 well plate
Reaction Speed: Standard
Detection Method: Primer-Probe Detection
Research Category: Stem Cell Research
Number of Reactions: 384 Reactions
For Use With (Equipment): ViiA™ 7 System, QuantStudio™ 12K Flex

Contents & storage

Contents: One 384-well plate with four replicates of the 96-assay panel and one optical cover

Store at room temperature.

Documents

Manuals & protocols