The LanthaScreen® TR-FRET ER beta Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) beta. The kit uses a human ER beta ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (Fluormone™ ES2) in a homogeneous mix-and-read assay format.
The Lanthascreen ER beta Competitive Binding Assay is:
• Complete & ready-to-use out of the box—all reagents included, just add your test compounds • High throughput compatible—TR-FRET assays in a 384-well plate • Convenient—components available in bulk sizes for larger screening needs
For Research Use Only. Not for use in diagnostic procedures.
Competitive Binding Assay, Nuclear Receptor Assay
Contents & storage
1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C) 1 tube of Fluormone ES2 (store at -5 to -30°C) 2 bottles NR Buffer (store at -5 to -30°C) 1 tube Tb-Anti-GST Ab (store at -5 to -30°C) 1 tube of DTT (store at -5 to -30°C)