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The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit. Improvements in the 2.0 system include increased efficiency, lower cytotoxicity, and faster clearance in the generation of induced pluripotent stem cells. This system continues to be easy to use and works with many different somatic cell types, using Sendai particles to deliver Yamanaka factors, which have been shown to be critical for efficient generation of iPSCs. This kit includes three vector preparations: polycistronic Klf4–Oct3/4–Sox2, cMyc, and Klf4.
Features of the CytoTune™-iPS 2.0 Sendai Reprogramming Kit:
• Ease of use—still requires only one application of the virus to obtain successful reprogramming
• Increased efficiency—usually at least 2-fold higher reprogramming efficiencies than the original kit
• Rapid clearance—CytoTune™-iPS products use the non-integrating Sendai virus that remains in the cytoplasm, reducing the likelihood of integration into the host genome. With the CytoTune™-iPS 2.0 Sendai virus, the Sendai backbone and transgenes clear the cytoplasm faster than the original.
The CytoTune™-iPS 2.0 Sendai system continues to be one of the easiest methods available for cell reprogramming. Only one application of the virus is required, instead of the multiple days of transductions required for mRNA-mediated reprogramming. It is also an ideal system for blood cell reprogramming.
The CytoTune™-iPS 2.0 Sendai Reprogramming Kit typically exhibits greater than 2-fold efficiency over the original kit, for both fibroblast and blood reprogramming. Efficiencies greater than 1% have been obtained with both fibroblasts and blood cells. (BJ and CD34+ cells, respectively).
Please refer to each kit's Certificate of Analysis for the appropriate titer and volume recommendations for reprogramming.