Invitrogen™

Fluorescent Protein Labeling Kits

Catalog number: A20170
Invitrogen™

Fluorescent Protein Labeling Kits

Catalog number: A20170
Ready to use in just 90 minutes, our protein labeling kits include an easy-to-use, pre-packed spin column for rapid dye removal and typical recovery greater than 85%. Each kit contains enough reagent for 3–5 protein conjugation reactions. Our kits provide better results due to lower background fluorescence, less nonspecific binding, and easier workflows for protein conjugation with 16 different fluorophores.
 
Catalog Number
A20170
also known as A-20170
Unit Size
1 kit
Description
Alexa Fluor™ 633 Protein Labeling Kit
Price (USD)
Price: 636.00
Online price: 610.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 633 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission633/647
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 633
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10170
also known as A-10170
Unit Size
1 kit
Description
Alexa Fluor™ 350 Protein Labeling Kit
Price (USD)
Price: 638.00
Online price: 612.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 350 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission346/442
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 350
ColorBlue
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10235
also known as A-10235
Unit Size
1 kit
Description
Alexa Fluor™ 488 Protein Labeling Kit
Price (USD)
Price: 648.00
Online price: 621.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 488 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission494/519
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10236
also known as A-10236
Unit Size
1 kit
Description
Alexa Fluor™ 532 Protein Labeling Kit
Price (USD)
Price: 600.00
Online price: 575.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 532 Protein Labeling Kit
Product TypeLabeling Kit
Quantity3 Reactions
Detection MethodFluorescence
Excitation/Emission530/554
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 532
ColorYellow
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10237
also known as A-10237
Unit Size
1 kit
Description
Alexa Fluor™ 546 Protein Labeling Kit
Price (USD)
Price: 640.00
Online price: 613.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 546 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission554/570
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 546
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 555 Protein Labeling Kit
Price (USD)
Price: 638.00
Online price: 612.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 555 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission553/565
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10238
also known as A-10238
Unit Size
1 kit
Description
Alexa Fluor™ 568 Protein Labeling Kit
Price (USD)
Price: 646.00
Online price: 619.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 568 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission577/603
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 568
ColorOrange-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A10239
also known as A-10239
Unit Size
1 kit
Description
Alexa Fluor™ 594 Protein Labeling Kit
Price (USD)
Price: 638.00
Online price: 612.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 594 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission590/617
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Description
Alexa Fluor™ 647 Protein Labeling Kit
Price (USD)
Price: 648.00
Online price: 621.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 647 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission650/668
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 647
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Catalog Number
A20171
also known as A-20171
Unit Size
1 kit
Description
Alexa Fluor™ 660 Protein Labeling Kit
Price (USD)
Price: 638.00
Online price: 612.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionAlexa Fluor™ 660 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission663/690
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 660
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Showing 10 of 15Show more
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
A20170
also known as A-20170
Full specifications
1 kitAlexa Fluor™ 633 Protein Labeling Kit
Price: 636.00
Online price: 610.65
Your price:
-
DescriptionAlexa Fluor™ 633 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission633/647
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 633
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10170
also known as A-10170
Full specifications
1 kitAlexa Fluor™ 350 Protein Labeling Kit
Price: 638.00
Online price: 612.65
Your price:
-
DescriptionAlexa Fluor™ 350 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission346/442
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 350
ColorBlue
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10235
also known as A-10235
Full specifications
1 kitAlexa Fluor™ 488 Protein Labeling Kit
Price: 648.00
Online price: 621.65
Your price:
-
DescriptionAlexa Fluor™ 488 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission494/519
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10236
also known as A-10236
Full specifications
1 kitAlexa Fluor™ 532 Protein Labeling Kit
Price: 600.00
Online price: 575.65
Your price:
-
DescriptionAlexa Fluor™ 532 Protein Labeling Kit
Product TypeLabeling Kit
Quantity3 Reactions
Detection MethodFluorescence
Excitation/Emission530/554
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 532
ColorYellow
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10237
also known as A-10237
Full specifications
1 kitAlexa Fluor™ 546 Protein Labeling Kit
Price: 640.00
Online price: 613.65
Your price:
-
DescriptionAlexa Fluor™ 546 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission554/570
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 546
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A20174Full specifications
1 kitAlexa Fluor™ 555 Protein Labeling Kit
Price: 638.00
Online price: 612.65
Your price:
-
DescriptionAlexa Fluor™ 555 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission553/565
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 555
ColorOrange
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10238
also known as A-10238
Full specifications
1 kitAlexa Fluor™ 568 Protein Labeling Kit
Price: 646.00
Online price: 619.65
Your price:
-
DescriptionAlexa Fluor™ 568 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission577/603
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 568
ColorOrange-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A10239
also known as A-10239
Full specifications
1 kitAlexa Fluor™ 594 Protein Labeling Kit
Price: 638.00
Online price: 612.65
Your price:
-
DescriptionAlexa Fluor™ 594 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission590/617
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 594
ColorRed
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A20173Full specifications
1 kitAlexa Fluor™ 647 Protein Labeling Kit
Price: 648.00
Online price: 621.65
Your price:
-
DescriptionAlexa Fluor™ 647 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission650/668
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 647
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
A20171
also known as A-20171
Full specifications
1 kitAlexa Fluor™ 660 Protein Labeling Kit
Price: 638.00
Online price: 612.65
Your price:
-
DescriptionAlexa Fluor™ 660 Protein Labeling Kit
Product TypeLabeling Kit
Detection MethodFluorescence
Excitation/Emission663/690
Labeling MethodConjugation-Based
Labeling Scale1 mg
Shipping ConditionRoom Temperature
Product LineAlexa Fluor™
Chemical ReactivityAmine
SolubilityDMSO (Dimethylsulfoxide)
Label or DyeAlexa Fluor 660
ColorFar-Red
Labeling TargetAntibodies, Proteins
Label TypeAlexa Fluor Dyes
Unit Size1 kit
Showing 10 of 15Show more
  • Fluorescently label up to 1 mg of protein per reaction (three reactions per kit)
  • Label 0.5–3 mg per reaction with DSB-X Biotin Protein Labeling Kit (five reactions per kit)
  • Labeled proteins ready to use in 90 min. (∼15 min. hands-on time)
  • Rapidly purify proteins by quickly removing unbound dye using pre-packed Zeba Dye and Biotin Removal Spin Columns (7K MWCO, Cat. No. A44299) for >85% recovery
  • Includes detailed instructions for determining degree of labeling (DOL)

Each protein labeling kit contains everything you need to perform 3-5 separate labeling reactions and purify the resulting conjugates. The reactive dye has either a succinimidyl ester (SE) or a tetrafluorophenyl (TFP) ester moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. Each of the vials of reactive dye provided in the kit is sufficient for labeling 1 mg of a variety of purified proteins, including growth factors, cytokines, nanobodies, enzymes, cell-adhesion molecules, and antibodies.

Direct labeling with fluorophores allows multiple primary antibodies of the same isotype (derived from the same species) to be used in the same experiment. Stabilizing proteins such as BSA should be removed from the sample before labeling.

The different protein labeling kits

  • Blue-fluorescent Alexa Fluor 350 —excitation and emission maxima of 346/442 nm
  • Green-fluorescent Alexa Fluor 488 – excitation and emission maxima of 494/519 nm; excited using a 488 nm argon laser line and detected under standard FITC/Cy2 filters
  • Yellow-fluorescent Alexa Fluor 532 – excitation and emission maxima of 530/554 nm; excited using a 532 nm Nd:YAG laser line and detected under standard Rhodamine 6G filters
  • Orange-fluorescent Alexa Fluor 546 – excitation and emission maxima of 554/570 nm; excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters
  • Orange-fluorescent Alexa Fluor 555 – excitation and emission maxima of 555/565 nm; excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters
  • Orange-red-fluorescent Alexa Fluor 568 – excitation and emission maxima of 577/603 nm; excited using a 568 nm Kr laser line and detected under standard Rhodamine Red/Cy3.5 filters
  • Red-fluorescent Alexa Fluor 594 – excitation and emission maxima of 590/617 nm; excited using a 594 nm Kr or He-Ne laser line and detected under standard Texas Red filters
  • Far-red-fluorescent Alexa Fluor 633 – excitation and emission maxima of 632/647 nm
  • Far-red-fluorescent Alexa Fluor 647 – excitation and emission maxima of 650/668 nm; excited using a 633 or 635 nm Kr or He-Ne laser line and detected under standard APC/Cy5 filters.
  • Far-red-fluorescent Alexa Fluor 660 – excitation and emission maxima of 663/690 nm
  • Near-IR-fluorescent Alexa Fluor 680 – excitation and emission maxima of 680/700 nm
  • Green-fluorescent Fluorescein-EX – excitation and emission maxima of 494/518 nm
  • Oregon Green 488 – excitation and emission maxima of 496/524 nm
  • Pacific Blue – a violet light excitable dye with an excitation and emission maxima of 410/455 nm
  • Texas Red-X – excitation and emission maxima of 595/615 nm
  • DSB-X Biotin – Conjugates can be reversibly bound to biotin-binding proteins such as streptavidin or avidin. The concentration (mg/mL) of the DSB-X Biotin-labeled antibody preparation can be determined by measuring the absorbance of the dialyzed sample at 280 nm and dividing this value by 1.3 or 1.4 when measured in solution in a cuvette with a 1-cm pathlength. DSB-X Biotin does not absorb significantly at 280 nm.

For labeling smaller amounts of antibodies (∼100 μg), we recommend our antibody labeling kits. Please review the protein labeling kit user manual for more in depth information, protocols, molecular weights, and degree of labeling for each dye.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in refrigerator 2°C to 8°C and protect from light.

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