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Invitrogen
Nitrotyrosine Polyclonal Antibody
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FIGURE: 1 / 20
Nitrotyrosine Antibody (A-21285) in ICC/IF
Nitrated tyrosine residues in bovine pulmonary artery endothelial cells detected with rabbit anti-nitrotyrosine antibody. Fixed and permeabilized bovine pulmonary artery endothelial cells were treated with either degraded peroxynitrite (top panel) or 100 µM peroxynitrite (bottom panel) for five minutes at room temperature to induce protein nitration. Nitrated tyrosine residues were detected with our rabbit ... View More
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.jpg?time=20240507?time=20240507 "Nitrotyrosine Antibody (A-21285) in ICC/IF")
.jpg?time=20240507?time=20240507 "Nitrotyrosine Antibody (A-21285) in IHC (P)")
Product Details
A-21285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Keyhole limpet hemocyanin (KLH) containing nitrated tyrosines
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from superoxide and nitric oxide (NO) radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g., alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and superoxide dismutase (SOD). Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious, and degenerative human diseases, such as Alzheimer's and Parkinson's disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis, and a variety of conditions precipitated by endothelial injury. At the protein level, tyrosine nitration can lead to loss or alteration of protein function, as demonstrated for the oncogenic protein p53 and the mitochondrial protein, manganese-dependent superoxide dismutase (MnSOD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nitro-tyrosine
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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