The RapidFinder™ Poultry ID Kit enables the simple and reliable detection of poultry meat in food and feed samples. The method works by detection of avian DNA, including DNA from chicken, turkey, duck, ostrich, goose, and other birds, by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Poultry ID Kit detects both raw and processed poultry meat and provides sensitivity down to 0.01% poultry DNA. The included positive control in the kit, which comprises 0.1% poultry DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-poultry DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Poultry ID Kit Works
Poultry DNA detection is accomplished by real-time PCR using the supplied primer/probe set targeting mitochondrial DNA sequence specific to several avian species, including but not limited to Gallus gallus (chicken), Meleagris gallopavo (turkey), Anas platyrhynchos (duck), Struthio camelus (ostrich), and Anser (goose). The primer/probes are supplied in a single tube, so if any of the poultry species is detected, a positive result will be obtained. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% Gallus gallus DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems™ StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Poultry ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Poultry ID Kit, most labs will go on to quantify the amount of poultry DNA in the sample. This can be accomplished using the RapidFinder™ Poultry ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Poultry ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, ruminant, chicken, and turkey.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).