Mouse (ICR) Inactivated Embryonic Fibroblasts

Mouse (ICR) Inactivated Embryonic Fibroblasts - FAQs

View additional product information for Mouse (ICR) Inactivated Embryonic Fibroblasts - FAQs (A24903)

3 product FAQs found

What is the difference between Mouse (ICR) Inactivated Embryonic Fibroblasts (Cat. No. A24903) and CF1 Mouse Embryonic Fibroblasts, irradiated (Cat. Nos. A34180, A34181)? Can they be used to reprogram fibroblasts?

Mouse (ICR) Inactivated Embryonic Fibroblasts and CF1 Mouse Embryonic Fibroblasts, irradiated are inactivated feeder layer fibroblast cells that are isolated from different mouse strains. Researchers often have a preference for one or the other. Neither of these can be used to reprogram fibroblasts.

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Does the culture vessel need to be coated prior to seeding Mouse (ICR) Inactivated Fibroblasts?

Yes, the cutlure vessel needs to be coated with Attachment Factor Protein (Cat. No. S006100) at 37 degrees C for 30 min or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.

What is the sex of mouse embryros from which Mouse (ICR) Inactivated Fibroblasts are isolated?

We do not determine the sex of the embryos. The embryos were pooled from several litters which are a mixture of male and female.