SILAC RPMI 1640 Flex Media, no glucose, no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: A2494201

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture

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Description

SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine. RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells. Cells successfully cultured in RPMI-1640 include HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of applications. Find the right modification using the media selector tool.
This RPMI 1640 Medium is modified as follows:
WithWithout
 • Glucose
 • Phenol red
 • HEPES
 • L-arginine
 • L-glutamine
 • L-lysine

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Glutamine: No Glutamine
Phenol Red Indicator: No Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: No Sodium Pyruvate
Glucose: No Glucose
Product Size: 500 mL
Volume (Metric): 500.0 mL
Green Features: Fewer resources used, Less waste

Contents & storage

Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.

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Manuals & protocols

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