Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma.
• Universal RT
—one RT step for all TaqMan Advanced miRNA Assays
—detect as few as 60 copies input into cDNA synthesis
—detect only the mature miRNA and distinguish between highly homologous targets
• Small sample input
—detect and quantify mature miRNA from as little as 1 pg total RNA or 2 µL plasma
with biofluids including human serum, plasma, and tissueTaqMan miRNA Assay selection guide
| ||TaqMan MicroRNA Assays||TaqMan Advanced miRNA Assays|
|Description||TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR||TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR|
|RT chemistry||miRNA-specific RT||Universal RT|
|Throughput||Best for 1–10 targets||Best for >10 targets|
|Coverage||205 species available, coverage for miRBase v.21||All human, mouse, and rat miRNAs; coverage for miRBase v.21|
For Research Use Only. Not for use in diagnostic procedures.