PowerUp™ SYBR® Green Master Mix

Catalog number: A25779

Applied Biosystems™  Related applications: Real Time PCR (qPCR)

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PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

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1 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
2 For optimal performance, standard cycling using the Applied Biosystems® 7900 system is recommended.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): 7300 System, 7700 System, 7500 Fast System, ViiA™ 7 System, Bio-Rad CFX384 Touch™, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, StepOnePlus™ System, 7500 Fast Dx System, Roche LightCycler 480, Bio-Rad CFX Connect™, 7900HT Fast System
Reaction Speed: Fast or Standard
PCR Method: qPCR
Sample Type (General): DNA
Polymerase: Dual-Lock™ Taq DNA Polymerase
Product Size: 2 x 1 mL
Shipping Condition: Wet Ice
Number of Reactions: 200 reactions

Contents & storage

The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 2 X 1 mL tube, sufficient for 200 20-µL reactions (10 µL Master Mix per reaction).

Store at 2–8°C.


Manuals & protocols

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