Gibco™

Essential 8™ Adaptation Kit

Catalog number:  A25935

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Description

The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately.

The Essential 8 Adaptation Kit is:
• Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Physiologically relevant–expand cells with minimal differentiation
• Scalable–passage stem cell lines as single cells with no impact to genetic stability

Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (iPS - Induced Pluripotent Stem)
Product Line: Essential 8™
Product Size: 1 kit
Shipping Condition: Dry Ice

Contents & storage

100 µg rhLaminin-521, store at -5 to -30°C
500 mL basal medium, store at 2-8°C
10 mL supplement, store at -5 to -30°C

Documents

Manuals & protocols