The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately.
The Essential 8 Adaptation Kit is: • Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort • Physiologically relevant–expand cells with minimal differentiation • Scalable–passage stem cell lines as single cells with no impact to genetic stability
Trouble-free transition When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.
Confident expansion rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.
Easy scale-up rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
For Research Use Only. Not for use in diagnostic procedures.