Detection of kappa opioid receptors on mouse thymocyte phenotypic subpopulations as assessed by flow cytometry.
AuthorsIgnatowski TA, Bidlack JM
JournalJ Pharmacol Exp Ther
PubMed ID9435191
'Recent studies have shown kappa opioid receptor labeling on the R1EGO thymoma cell line by indirect immunofluorescence and flow cytometric analysis. The present study used a fluorescein-labeled arylacetamide (FITC-AA), a kappa opioid ligand, in conjunction with biotin-conjugated anti-fluorescein IgG and extravidin-R-phycoerythrin (PE), along with double-labeling with antibodies against specific immune ... More
A complementary role for the tetraspanins CD37 and Tssc6 in cellular immunity.
AuthorsGartlan KH, Belz GT, Tarrant JM, Minigo G, Katsara M, Sheng KC, Sofi M, van Spriel AB, Apostolopoulos V, Plebanski M, Robb L, Wright MD,
JournalJ Immunol
PubMed ID20709950
'The cooperative nature of tetraspanin-tetraspanin interactions in membrane organization suggests functional overlap is likely to be important in tetraspanin biology. Previous functional studies of the tetraspanins CD37 and Tssc6 in the immune system found that both CD37 and Tssc6 regulate T cell proliferative responses in vitro. CD37(-/-) mice also displayed ... More
Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.
Authorsvan Roy N, Mangelschots K, Speleman F
JournalTrends Genet
PubMed ID8488564
Isolating and engineering human antibodies using yeast surface display.
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display. Single-chain antibody fragments (scFvs) are first isolated from an existing nonimmune human library displayed on the yeast surface using magnetic-activated cell sorting selection followed by selection using flow cytometry. ... More
High-throughput single-molecule spectroscopy in free solution.
AuthorsMa Y, Shortreed MR, Yeung ES
JournalAnal Chem
PubMed ID11028623
A high-speed high-throughput single-molecule imaging technique for identifying molecules in free solution based on differences in their fluorescence emission spectra is presented. Unlike previous reports, the entire spectrum, rather than selected wavelengths through optical filters, is recorded. Furthermore, the millisecond data acquisition time means that the molecules do not need ... More
AuthorsSiiman O, Wilkinson J, Burshteyn A, Roth P, Ledis S
JournalBioconjug Chem
PubMed ID10563780
New, highly amino-substituted dextran or aminodextran (hereafter denoted Amdex) of various sizes between about 20 and 1000 kDa molecular mass and degrees of amino-substitution between 7 and 40% were prepared and characterized by elemental analyses and polyacrylamide gel electrophoresis. These aminodextrans together with others commercially available were shown by static ... More
Fluorescent staining of kappa opioid receptors using naltrexamine derivatives and phycoerythrin.
AuthorsLawrence DM, Hutchinson I, Seyed-Mozaffari A, Archer S, Bidlack JM
JournalJ Immunol Methods
PubMed ID9050939
An immunofluorescent technique that is more sensitive than radioligand binding was developed in order to detect opioid receptors expressed on leukocytes. The current study was designed to optimize the method for fluorescently labeling kappa opioid receptors. For these experiments, the opioid antagonist naltrexamine was conjugated to either fluorescein (FITC-NTXamine) or ... More