Thermo Scientific™

High Select™ Phosphopeptide Enrichment Kits & Reagents

Catalog number: A32992
Thermo Scientific™

High Select™ Phosphopeptide Enrichment Kits & Reagents

Catalog number: A32992
Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionization, and incomplete fragmentation of phosphopeptides. Given the low relative abundance of phosphorylation modifications in complex protein samples, enrichment is essential for successful MS analysis of phosphopeptides. High-Select Fe-NTA and TiO2 phosphopeptide enrichment kits complement our lysis, reduction, alkylation, and digestion reagents, along with our C18, graphite spin, and high-pH reversed-phase peptide fractionation columns to provide a complete workflow for phosphopeptide enrichment.
 
Catalog Number
A32992
Unit Size
30 reactions
Product Line
High Select™
Description
Fe-NTA Phosphopeptide Enrichment Kit
Price (USD)
Price: 425.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionFe-NTA Phosphopeptide Enrichment Kit
For Use With (Equipment)Microcentrifuge
Product TypePhosphopeptide Enrichment Kit
Product LineHigh Select™
Label or DyeUnlabeled
Shipping ConditionWet Ice
Starting MaterialProtease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatKit
Workflow StepPeptide Enrichment
Quantity30 Reactions
Unit Size30 reactions
Catalog Number
Unit Size
20 reactions
Product Line
High Select™
Description
Fe-NTA Magnetic Phosphopeptide Enrichment Kit
Price (USD)
Price: 455.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionFe-NTA Magnetic Phosphopeptide Enrichment Kit
Product LineHigh Select™
Quantity0.5 mL
Unit Size20 reactions
Catalog Number
Unit Size
5 mL
Product Line
High Select™
Description
Fe-NTA Magnetic Agarose
Price (USD)
Price: 294.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionFe-NTA Magnetic Agarose
Product LineHigh Select™
Quantity5 mL
Unit Size5 mL
Catalog Number
Unit Size
24 reactions
Product Line
High Select™
Description
TiO2 Phosphopeptide Enrichment Kit
Price (USD)
Price: 590.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionTiO2 Phosphopeptide Enrichment Kit
For Use With (Equipment)Microcentrifuge
Product TypePhosphopeptide Enrichment Kit
Product LineHigh Select™
Label or DyeUnlabeled
Shipping ConditionWet Ice
Starting MaterialProtease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatKit
Workflow StepPeptide Enrichment
Quantity24 Reactions
Unit Size24 reactions
Catalog Number
Unit Size
30 columns
Product Line
Pierce™
Description
Graphite Spin Columns
Price (USD)
Price: 165.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionGraphite Spin Columns
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Clean Up
Quantity30 Columns
Unit Size30 columns
Catalog Number
Unit Size
96 tips
Product Line
Pierce™
Description
TiO2 Phosphopeptide Enrichment Tips
Price (USD)
Price: 1,458.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionTiO2 Phosphopeptide Enrichment Tips
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePhosphopeptide Enrichment Spin Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Enrichment
Quantity96 Tips
Unit Size96 tips
Showing 6 of 6
Catalog NumberSpecificationsUnit SizeProduct LineDescriptionPrice (USD)AvailabilityQuantity
A32992Full specifications
30 reactionsHigh Select™Fe-NTA Phosphopeptide Enrichment Kit
Price: 425.00
Your Price:
-
DescriptionFe-NTA Phosphopeptide Enrichment Kit
For Use With (Equipment)Microcentrifuge
Product TypePhosphopeptide Enrichment Kit
Product LineHigh Select™
Label or DyeUnlabeled
Shipping ConditionWet Ice
Starting MaterialProtease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatKit
Workflow StepPeptide Enrichment
Quantity30 Reactions
Unit Size30 reactions
A52283Full specifications
20 reactionsHigh Select™Fe-NTA Magnetic Phosphopeptide Enrichment Kit
Price: 455.00
Your Price:
-
DescriptionFe-NTA Magnetic Phosphopeptide Enrichment Kit
Product LineHigh Select™
Quantity0.5 mL
Unit Size20 reactions
A52284Full specifications
5 mLHigh Select™Fe-NTA Magnetic Agarose
Price: 294.00
Your Price:
-
DescriptionFe-NTA Magnetic Agarose
Product LineHigh Select™
Quantity5 mL
Unit Size5 mL
A32993Full specifications
24 reactionsHigh Select™TiO2 Phosphopeptide Enrichment Kit
Price: 590.00
Your Price:
-
DescriptionTiO2 Phosphopeptide Enrichment Kit
For Use With (Equipment)Microcentrifuge
Product TypePhosphopeptide Enrichment Kit
Product LineHigh Select™
Label or DyeUnlabeled
Shipping ConditionWet Ice
Starting MaterialProtease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatKit
Workflow StepPeptide Enrichment
Quantity24 Reactions
Unit Size24 reactions
88302Full specifications
30 columnsPierce™Graphite Spin Columns
Price: 165.00
Your Price:
-
DescriptionGraphite Spin Columns
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypeColumn
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Clean Up
Quantity30 Columns
Unit Size30 columns
88303Full specifications
96 tipsPierce™TiO2 Phosphopeptide Enrichment Tips
Price: 1,458.00
Your Price:
-
DescriptionTiO2 Phosphopeptide Enrichment Tips
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Product TypePhosphopeptide Enrichment Spin Tip
Product LinePierce™
Starting MaterialPeptides, Protease-digested Protein
Final Product TypePeptides
Detection MethodMass Spectrometry
FormatSpin Column
Workflow StepSample Enrichment
Quantity96 Tips
Unit Size96 tips
Showing 6 of 6
Convenient—pre-formulated buffers for ease-of-use in spin-column (Fe-NTA and TiO2) or magnetic (Fe-NTA) formats to enable parallel processing of multiple samples
High specificity—recovers phosphopeptides with > 90% selectivity
Superior recovery—enriches more total and unique phosphopeptides than other commercially available resins
High coverage—recovers peptides with single and multiple phosphorylation sites
Scalable—compatible with automation, including the use of KingFisher magnetic particle processors

High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest as starting sample. Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150 µg of phosphopeptides with greater than 90% selectivity in less than 45 minutes.

High-Select TiO2 Phosphopeptide Enrichment Kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides. Each column can enrich phosphopeptides from 0.5 to 3 mg of total protein digest as starting sample. The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns. Use of this kit results in enrichment of up to 150 µg of phosphopeptides with greater than 90% selectivity. TiO2 enriches a unique set of phosphopeptides that complements the High-Select Fe-NTA IMAC Phosphopeptide Enrichment Kit.

High-Select Fe-NTA Magnetic Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate magnetic agarose beads. Each reaction is sufficient to enrich 250–1000 µg of phosphopeptides from 0.5–5 mg of total protein digest as starting sample in less than 45 minutes. Additionally, the kit may also be used to enrich for chemically modified peptides with functional groups designed for use with immobilized metal affinity chromatography resins.

High Select Fe-NTA Magnetic Agarose has been specifically designed to enrich phosphopeptides and chemically modified peptides for MS analysis. Our non-aggregating, magnetite (Fe3O4), superparamagnetic beads have exceptional uniformity for high performance using both manual and automated purification applications. The Fe-NTA Magnetic agarose is available separately to provide flexibility in experimental design.

For high resolution analysis of the enriched phosphopeptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat. No. ES902 or ES903). The stand-alone High Select Fe-NTA Magnetic Agarose beads provide flexibility for scale and throughput and may be used in both manual and automated platforms, including KingFisher 96 and KingFisher Flex magnetic particle processors.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

• Spin columns (30)
• White Luer plug (end cap) (30)
• Binding/Wash Buffer (2 x 20 mL)
• Phosphopeptide Elution Buffer (7 mL)