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High Select™ HSA/Immunoglobulin Depletion Mini Spin Columns, 24 Columns - FAQs

High Select™ HSA/Immunoglobulin Depletion Mini Spin Columns, 24 Columns - FAQs

View additional product information for High Select™ Depletion Spin Columns - FAQs (A36369, A36368, A36367, A36372, A36371, A36370, A36366, A36365)

8 product FAQs found

After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?

Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?

After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the maximum loading capacity for the High Select Depletion columns?

Mini columns can deplete up to 10 µL (600 µg) of plasma or serum; midi columns can deplete up to 100 µL (6000 µg) of plasma or serum.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the percentage of albumin that is removed using abundant protein depletion columns?

More than 99% of albumin can be depleted when columns use the recommended sample to resin ratio.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I assess efficiency and reproducibility of abundant protein depletion in plasma samples?

ELISA and mass spectrometry analysis are often used for evaluation of depletion performance. Protein yields after depletion can be measured using the BCA assay.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I use the High Select HSA/Immunoglobulin Depletion Resin to process mouse serum samples?

No, the High Select HSA/Immunoglobulin Depletion Resin cannot be used for mouse samples; the depletion resin contains immobilized antibodies that only recognize human serum or plasma proteins.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I reuse the High Select HSA/Immunoglobulin Depletion Resin?

We don’t recommend reusing the High Select HSA/Immunoglobulin Depletion Resin as depletion efficiency may decrease after regeneration, and subsequent depleted samples may be contaminated from prior ones.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of the High Select HSA/Immunoglobulin Depletion Resin do you offer?

The High Select HSA/Immunoglobulin Depletion Resin is offered in 3 formats:

a) Resin alone: High Select HSA/Immunoglobulin Depletion Resin (Cat. No. A36368)
b) High Select HSA/Immunoglobulin Depletion Mini Spin Columns (Cat. Nos. A36365, A36366) that can process a maximum of 10 µL of human serum or plasma
c) High Select HSA/Immunoglobulin Depletion Midi Spin Columns (Cat. No. A36367) that can process a maximum of 100 µL of human serum or plasma. For serum samples containing abnormally high amounts of albumin or IgG, the sample load may need to be reduced.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.