Sanger Sequencing Kit - FAQs

When using the Sanger Sequencing Kit, we recommend using a sequencing reaction volume of 10 µL.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

For the sequencing reaction, we recommend using 3 µL of purified PCR product (purified using ExoSAP-IT Express PCR Product Cleanup Reagent from the kit).

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

The Sanger Sequencing Kit includes the following components:
- ExoSAP-IT Express PCR Product Cleanup Reagent, which removes excess primers and unincorporated nucleotides from a PCR reaction
- BigDye Terminator v3.1 Cycle Sequencing Kit, which provides premixed reagents for Sanger sequencing reactions
- BigDye XTerminator Purification Kit, a fast, simple purification method for DNA sequencing reactions, that removes unincorporated BigDye terminators and salts

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Yes, all the components in the Sanger Sequencing Kit are available separately. The individual components can be found on the following product pages:
- For the ExoSAP-IT Express PCR Product Cleanup Reagent please go here (https://www.thermofisher.com/order/catalog/product/75001.4X.1.ML). We recommend replacing the strip septa after each run. Bleach, detergents, or solvents should not be used to clean the septa. Use of these reagents can cause them to dry out faster and risk contaminating the system.
- For the BigDye Terminator v3.1 Cycle Sequencing Kit, please go here (https://www.thermofisher.com/order/catalog/product/4337454).
- For the BigDye XTerminator Purification Kit, please go here (https://www.thermofisher.com/order/catalog/product/4376486).

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

The Sanger Sequencing Kit is designed for our complete sequencing workflow on the SeqStudio, 3500 series, and 3730 series genetic analyzers. The new kit provides all the components needed to clean up a PCR product for sequencing, perform cycle sequencing, and then purify the sequencing product. This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.