The TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control is a synthetic RNA control that serves as an exogenous process control for nucleic acid isolation and RNA recovery, reverse transcription, pre-amplification, and PCR. The control is used with the proprietary TaqMan assay for Xeno sequences (Assay IDs Ac00010014_a1 or Pa00010014_a1).
The TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control is supplied at a concentration of 10,000 copies/µL. During nucleic acid isolation, 10 µL of the control is added to each test sample along with the nucleic acid binding reagents. The control is used to identify sample-specific amplification inhibition, reducing the likelihood of false negatives and providing confidence that results are accurate.