B-27™ Plus Neuronal Culture System with Neurobasal™ PhysioPlus Medium, reduced glucose, no additives - FAQs

Gibco Neurobasal PhysioPlus Medium has a shelf life of 12 months from the date of manufacture when stored at 4°C and protected from light. After supplementation with Gibco B-27 Plus Supplement, store complete medium at 4°C protected from light and use within 2-4 weeks.

Gibco Neurobasal PhysioPlus Medium contains 6 mM glucose and 0.5 mM Gibco GlutaMAX Supplement.

Gibco Neurobasal PhysioPlus Medium is formulated with a reduced glucose concentration to more closely approximate physiological brain extracellular levels. Researchers requiring higher glucose concentrations may prefer other Neurobasal formulations depending on their application. If a medium completely free of glucose is needed, we offer Gibco Neurobasal-A Medium, no D-glucose, no sodium pyruvate.

Use Gibco B-27 Plus Supplement with Gibco Neurobasal PhysioPlus Medium.

Gradual adaptation to Gibco Neurobasal PhysioPlus Medium is typically not required. You can transition during a routine media change by replacing half the media volume with Neurobasal PhysioPlus Medium. We recommend verifying performance in your specific neuronal model when modifying an established protocol.

Use Gibco Neurobasal PhysioPlus Medium for neuronal maintenance and maturation after differentiation is complete. Continue your established differentiation protocol, then transition to Neurobasal PhysioPlus Medium with B-27 Plus Supplement when the culture consists predominantly of post-mitotic neurons displaying neuronal morphology and active differentiation has largely completed.

Yes. Gibco Neurobasal PhysioPlus Medium can be used with three-dimensional neuronal models, including organoids and assembloids. We recommend introducing Neurobasal PhysioPlus Medium with B-27 Plus Supplement during maintenance, after differentiation is complete, and optimizing conditions for the specific model.

Yes. Gibco Neurobasal PhysioPlus Medium can be used for co-culture of neurons and glial cells. Optimization may be needed based on the additional cell types and their media requirements. We recommend verifying conditions for each specific co-culture model.

Yes. Gibco Neurobasal PhysioPlus Medium has been demonstrated to support primary rodent neurons and multiple human PSC-derived neuronal models. Separate user manuals are available for primary neuron and iPSC-derived neuron applications.