Vitronectin (VTN-N) Recombinant Human Protein, truncated

Vitronectin (VTN-N) is a recombinant human protein that offers a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8 Medium (Cat. No. A1517001), vitronectin has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines. Vitronectin (VTN-N) has been shown to support PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages.

Vitronectin (VTN-N) is:

• Optimized—designed in the laboratory of James Thomson for use with Essential 8 Medium
• Defined—for reduced variability in your PSC cultures
• Cost effective—for economical and scalable PSC culture

Optimized and defined surface

Unlike standard basement membrane extracts (BMEs), vitronectin (VTN-N) is based on the N-variant design of vitronectin from the laboratory of James Thomson for use with Essential 8 Medium. The vitronectin (VTN-N) variant has been shown to support human pluripotent stem cell attachment and survival better than wild type vitronectin. Vitronectin (VTN-N) helps provide an optimal environment for feeder-free pluripotent stem cell culture.

Reduced variability

Vitronectin (VTN-N) is a defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs).

Cost effective

When used with Essential 8 Medium, vitronectin (VTN-N) provides a cost effective, defined system for feeder free culture of human pluripotent stem cells (PSCs).

Commercialized in Partnership with Cellular Dynamics International.

Reference

(1) Chen G, et al. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 8(5):424-9.