Applied Biosystems™

resDNASEQ™ Quantitative Vero DNA Kits

Catalog number: A41797
Have Questions?
Applied Biosystems™

resDNASEQ™ Quantitative Vero DNA Kits

Catalog number: A41797
Have Questions?
The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types.
 
Catalog Number
A41797
Unit Size
100 reactions
Includes
Assay Kit only
Price (USD)
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellVero
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
Catalog Number
Unit Size
100 reactions
Includes
Assay Kit and Sample Preparation
Price (USD)
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellVero
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeIncludesPrice (USD)AvailabilityQuantity
A41797Full specifications
100 reactionsAssay Kit only
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellVero
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
A45522Full specifications
100 reactionsAssay Kit and Sample Preparation
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellVero
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Features of the resDNASEQ Quantitative Vero DNA Kit include:
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Offers consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero genomic DNA standard
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices

This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.

The resDNASEQ Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.

Consistent performance even with fragmented DNA
For accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

resDNASEQ Vero DNA Control
• Vero DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ Vero DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• Vero DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

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