Oncomine Focus Assay, Chef-Ready Library

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. It provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single workflow. For a kit that includes reagents for manual library preparation, please see the Oncomine Focus Assay, AmpliSeq Library.

The Oncomine Focus Assay, Chef-Ready Library, is for the Ion Chef System and Ion GeneStudio S5 System workflow. It contains all the reagents needed to prepare libraries for 32 samples:

• Oncomine Focus Assay, DNA Chef-Ready panel—contains the DNA primer pool
• Oncomine Focus Assay, RNA Chef-Ready panel—contains the RNA primer pool
• Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System

From sample to answer
The Oncomine Focus Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.Learn more about Oncomine Focus Assays and Oncomine Oncology.