IgG1-Fc Recombinant Human Protein

Catalog number:  A42560

Related applications:

Protein Assays and Analysis

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Description

Immunoglobulin gamma-1 heavy chain constant region, partial (IgG1-Fc), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein standard and in cell biology research applications.

This recombinant human protein is expressed from a DNA sequence encoding the human IgG1-Fc region (AAC82527.1), containing residues 99 to 330, including a 103 Cys/Ser mutation.

N-terminal Sequence Analysis: Glu 99.

Activity:
1. Measured by its ability to bind human CD16a-His (Cat:10389-H08H1) in a functional ELISA.
2. Measured by its ability to bind human CD16a-AVI-His (Cat:10389-H27H) in a functional ELISA.
3.Measured by its ability to bind human CD16a-His (Cat:10389-H08H) in a functional ELISA.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

You may also be interested in the following IgG1 products:
ELISA Kit
Primary Antibodies
Protein Labeling Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Form: Lyophilized
Protein: Fc region of human immunoglobulin G1
Protein Form: Recombinant, Truncated
Research Category: Immunology, Inflammation
Shipping Condition: Room Temperature
Species: Human
Molecular Weight: 26 kDa
Purity: ≥ 95% by SDS-PAGE
Protein Subtype: Other Proteins
Gene Alias: Fc region of human immunoglobulin G1
Gene Symbol: IgG1-Fc
Validated Application: Assay Standard
Protein Family: Growth Factors & Receptors
Expression System: Mammalian
Protein Tag: IgG1 Fc Tag

Contents & storage

Store at -20 to -70 °C