TNF (TNFA) Recombinant Cynomolgus Protein

Catalog number:  A42630

Related applications:

Protein Assays and Analysis

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Description

Tumor Necrosis Factor (TNF), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the soluble form of cynomolgus TNF-alpha (Val 77-Leu 233) fused to an initial Met at the N-terminus.

Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 is typically 20-80 pg/ml.

Formulation: Lyophilized in 50 mM Tris, 50 mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Expression: E. coli
Form: Lyophilized
Gene Aliases: TNF-alpha
Gene Symbol: TNF
Molecular Weight: 17.4 kDa
Product Size: 5 µg
Protein Family: Cytokines & Receptors
Protein Name: tumor necrosis factor
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: Untagged
Purity: ≥ 90% by SDS-PAGE
Research Category: Apoptosis,Cell Cycle & Proliferation,Inflammation,Oncology
Species: Monkey
Type: CatalogProductData
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.