Applied Biosystems™

PowerTrack™ SYBR Green Master Mix

Catalog number:  A46110

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Description

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up. Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

Features of PowerTrack SYBR Green Master Mix include:
• Built-in visual indicators aid in reaction setup
• Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization
• Specific and tight reproducibility in Ct’s over a broad dynamic range
• Formulated with UNG/dUTP to prevent contamination of carryover PCR products

Built-in visual indicators
PowerTrack SYBR Green Master Mix contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well. A separate sample buffer is provided that contains an inert yellow dye. The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes. The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with PowerTrack SYBR Green Master Mix.

Formulated for maximum specificity and reproducibility
PowerTrack SYBR Green Master Mix contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification. PowerTrack SYBR Green Master Mix is designed for exceptional performance in your most challenging real-time PCR applications.

Broad instrument compatibility
PowerTrack SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments. It is also compatible with the Bio-Rad CFX96™, CFX384™, and iQ™5; Roche LightCycler™ 480; and Agilent Mx3005P™ systems. For optimal results, primer concentrations should be in the range of 300–800 nM.

UNG for carryover contamination control
Contamination in labs that routinely run PCR is a major concern for many researchers and is the source of most false positives. The inclusion of UNG and dUTP in PowerTrack SYBR Green Master Mix results in the degradation of any previously amplified PCR products and helps prevent contamination of subsequent qPCR reactions.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, QuantStudio™, Real-Time PCR Systems or Thermal Cyclers, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Product Line: Applied Biosystems™
Quantity: 2 x 5 mL
Sample Type: DNA
Shipping Condition: Dry Ice
Storage Requirements: -25°C to -15°C
Detection Method: SYBR
For Use With (Application): Real Time PCR (qPCR)
PCR Method: qPCR
Reaction Speed: Fast or Standard

Contents & storage

• PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.